Biological dosimetry after H2O2/L-histidine treatment

Michael Hausmann; Heiko Lentfer; Dietmar Wolf; Eckhard Bauer; Klaus Aldinger; Karl-Otto Greulich; Christoph G. Cremer

doi:10.1117/12.301128

1 January 1998 Biological dosimetry after H₂O₂/L-histidine treatment

Michael Hausmann, Heiko Lentfer, Dietmar Wolf, Eckhard Bauer, Klaus Aldinger, Karl-Otto Greulich, Christoph G. Cremer

Proceedings Volume 3199, Biomedical Systems and Technologies II; (1998) https://doi.org/10.1117/12.301128
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

In biological dosimetry after radiation or chemical exposure, it has been well established to estimate exposure doses from the relative rate of aberrant chromosomes, especially dicentric chromosomes in a given number of cells. For this purpose, dose-efficiency curves depending on laboratory parameters (e.g. preparation technique, analysis procedure etc.) have to be measured under standard conditions. For statistical reasons, a high number of chromosomes or cells, respectively, has to be evaluated. For a Chinese hamster cell line (CO60) as a typical model system in mutation research, a dose efficiency relation after H₂O₂/L-histidine treatment of the cells was determined using the Heidelberg slit-scan flow fluorometer. This technique has the advantage that several thousand chromosomes can be automatically analyzed in a very short time. As expected, for low doses of H₂O₂/L-histidine exposure, a nearly linear dependence of the relative number of dicentric chromosomes to the concentration of H₂O₂ was obtained. In order to correlate the relative number of dicentric chromosomes to the relative number of double strand breaks, the cells were analyzed by the technique of the neutral comet assay. The dose dependent `tail moment' obtained from the comet assay also showed a linear behavior. This confirmed the results obtained by slit-scan flow fluorometry. Furthermore, the linear dependence of the dose efficiency curve was well compatible to results obtained by visual counting by means of a fluorescence microscope. In this case chromosome 1 of the Chinese hamster cell line DON was specifically labelled by fluorescence in situ hybridization.

Citation Download Citation

Michael Hausmann, Heiko Lentfer, Dietmar Wolf, Eckhard Bauer, Klaus Aldinger, Karl-Otto Greulich, and Christoph G. Cremer "Biological dosimetry after H₂O₂/L-histidine treatment", Proc. SPIE 3199, Biomedical Systems and Technologies II, (1 January 1998); https://doi.org/10.1117/12.301128

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