Kinetics of zona pellucida thermodissolution in mouse zygotes

Laurent Descloux; Sohi Rastegar; Guy P. Delacretaz; Artha J. Hollis; Klaus Rink

doi:10.1117/12.297888

14 January 1998 Kinetics of zona pellucida thermodissolution in mouse zygotes

Laurent Descloux, Sohi Rastegar, Guy P. Delacretaz, Artha J. Hollis, Klaus Rink

Proceedings Volume 3195, Laser-Tissue Interaction, Tissue Optics, and Laser Welding III; (1998) https://doi.org/10.1117/12.297888
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

Zona Pellucida (ZP), the outer protective layer of mammalian eggs, is composed of glycoproteins that can be dissolved by temperature elevation. The kinetics of this thermo-dissolution process can play an important role in understanding the mechanism of photothermolysis by a laser. While a temperature of around 70 degrees Celsius has been previously reported as the critical dissolution temperature for the ZP, we found in this study that dissolution temperature has a wider range and depends on duration of exposure which suggests a kinetic rate process. In this paper we report the results of our measurements at different prescribed temperature elevations and subsequent calculations of the kinetics constants using an Arrhenius model.

Citation Download Citation

Laurent Descloux, Sohi Rastegar, Guy P. Delacretaz, Artha J. Hollis, and Klaus Rink "Kinetics of zona pellucida thermodissolution in mouse zygotes", Proc. SPIE 3195, Laser-Tissue Interaction, Tissue Optics, and Laser Welding III, (14 January 1998); https://doi.org/10.1117/12.297888

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