805-nm diode-laser-induced release of liposome-encapsulated dye for quantification of chorioretinal thermal damage: in-vivo study on a rabbit eye model

Serge R. Mordon; Thomas Desmettre; Jean-Marie Devoisselle; Sylvie Begu; Valerie A. Mitchell

doi:10.1117/12.297897

14 January 1998 805-nm diode-laser-induced release of liposome-encapsulated dye for quantification of chorioretinal thermal damage: in-vivo study on a rabbit eye model

Serge R. Mordon, Thomas Desmettre, Jean-Marie Devoisselle, Sylvie Begu, Valerie A. Mitchell

Author Affiliations +

Proceedings Volume 3195, Laser-Tissue Interaction, Tissue Optics, and Laser Welding III; (1998) https://doi.org/10.1117/12.297897
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

This study aimed to evaluate the feasibility of retinal thermal damage assessment in a rabbit eye model by using laser-induced release of liposome-encapsulated dye. After anethesia, thermosensitive liposomes (DSPC) loaded with 5,6- Carboxyfluorescein were injected intravenously to pigmented rabbits. Retinal photocoagulation were performed with a 810 nm diode laser (P equals 100 to 400 mW, (Phi) equals 500 micrometer, ls). Fluorescence measurements in the area of the laser exposures were then realized with a digitized angiograph. Histology was performed on retinal tissue and adjacent ares. Fluorescent spots were observed for fluences ranging from 50 plus or minus 10 J/cm² to 210 plus or minus 10 J/cm². The fluorescence intensity increased linearly with the fluence and reached a plateau at 150 plus or minus 10 J/cm². The fluorescence intensity was correlated to the maximum temperature at the center of the laser spot with a linear increase from 42 plus or minus 3 degrees Celsius to 65 plus or minus 3 degrees Celsius. The chorioretinal thermal damage quantified by histology was correlated to temperature. These results are in agreement with our two previous studies with DSPC liposomes for temperature measurements in a tissue model and then in a vascular model. In conclusion, this study demonstrates the possibility of a laser-induced release of liposome-encapsulated dye for a quantification of diode laser induced thermal damage in ophthalmology. Such a method could be useful for a real-time monitoring of laser photocoagulation for condition such as choroidal neovessels when a precise thermal damage is required near the foveolar area.

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Serge R. Mordon, Thomas Desmettre, Jean-Marie Devoisselle, Sylvie Begu, and Valerie A. Mitchell "805-nm diode-laser-induced release of liposome-encapsulated dye for quantification of chorioretinal thermal damage: in-vivo study on a rabbit eye model", Proc. SPIE 3195, Laser-Tissue Interaction, Tissue Optics, and Laser Welding III, (14 January 1998); https://doi.org/10.1117/12.297897

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KEYWORDS

Luminescence

Semiconductor lasers

Retina

Laser coagulation

Eye models

Ophthalmology

Temperature metrology

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