Paper
1 January 1998 Changes in cytochrome-oxidase oxidation in the occipital cortex during visual simulation: improvement in sensitivity by the determination of the wavelength dependence of the differential pathlength
Matthias Kohl-Bareis, Christian Nolte, Hauke R. Heekeren, Susanne Horst, J. Scholz, Hellmuth Obrig, Arno Villringer M.D.
Author Affiliations +
Abstract
In this study we assess changes in the hemoglobin oxygenation (oxy-Hb, deoxy-Hb) and the Cytochrome-C-Oxidase redox state (Cyt-ox) in the occipital cortex during visual stimulation by near infrared spectroscopy. For the calculation of changes in oxy-Hb, deoxy-Hb and Cyt-ox from attenuation data via a modified Beer-Lambert equation, the wavelength dependence of the differential pathlength factor (DPF), i.e. the ratio of the mean optical pathlength and the physical light-source-detector separation, has to be taken into account. The wavelength dependence of the DPF determines the crosstalk between the different concentrations and is therefore essential for a high sensitivity. Here a simple method is suggested to estimate the wavelength dependence of the DPF((lambda) ) from pulse induced attenuation changes measured on the head of adult humans. The essence is that the DPF is the ratio of the attenuation changes over absorption coefficient changes and the spectral form of the pulse correlated absorption coefficient change is proportional to the extinction coefficient of blood. Indicators for the validity of the DPF((lambda) ) derived for wavelengths between 700 and 970 nm are the stability of the calculated oxy-Hb, deoxy-Hb and Cyt-ox signals with variations of the wavelength range included for their calculation and its overall agreement with the data available from the literature. The DPF derived from pulse measurements was used for the analysis of attenuation data from cortical stimulations. We show that Cyt-ox in the occipital cortex of human subjects is transiently oxidized during visual stimulation.
© (1998) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Matthias Kohl-Bareis, Christian Nolte, Hauke R. Heekeren, Susanne Horst, J. Scholz, Hellmuth Obrig, and Arno Villringer M.D. "Changes in cytochrome-oxidase oxidation in the occipital cortex during visual simulation: improvement in sensitivity by the determination of the wavelength dependence of the differential pathlength", Proc. SPIE 3194, Photon Propagation in Tissues III, (1 January 1998); https://doi.org/10.1117/12.301055
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Cited by 10 scholarly publications and 1 patent.
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KEYWORDS
Signal attenuation

Visualization

Oxidation

Absorption

Blood

Head

Human subjects

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