Induction of endogenic porphyrin production in bacteria and subsequent photoinactivation by various light sources

Yeshayahu Nitzan; Zvi Malik; Merav Kauffman; Benjamin Ehrenberg

doi:10.1117/12.297789

29 December 1997 Induction of endogenic porphyrin production in bacteria and subsequent photoinactivation by various light sources

Yeshayahu Nitzan, Zvi Malik, Merav Kauffman, Benjamin Ehrenberg

Author Affiliations +

Proceedings Volume 3191, Photochemotherapy: Photodynamic Therapy and Other Modalities III; (1997) https://doi.org/10.1117/12.297789
Event: BiOS Europe '97, 1997, San Remo, Italy

Abstract

(delta) -aminolevulinic acid (ALA) induces the production of very high amounts of porphyrins in Gram-positive and Gram- negative bacteria. Accumulation of the porphyrins in the bacterial cell is a consequence of the high porphyrin production but most of the porphyrins are excreted from the cells into the medium. By fluorescence, measurements of the endogenic and of the exogenic content of the produced porphyrins can be determined. Bacteria loaded by their own accumulated porphyrins can undergo photoinactivation by various light sources. Killing of S. aureus cells by its endogenic porphyrins can be achieved by illumination with intense blue lights or by HeNe laser. E. coli cells loaded with endogenic porphyrins can be photoinactivated by intense blue and red light.

Citation Download Citation

Yeshayahu Nitzan, Zvi Malik, Merav Kauffman, and Benjamin Ehrenberg "Induction of endogenic porphyrin production in bacteria and subsequent photoinactivation by various light sources", Proc. SPIE 3191, Photochemotherapy: Photodynamic Therapy and Other Modalities III, (29 December 1997); https://doi.org/10.1117/12.297789

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