Neurotransmitter measurement with a fiber optic probe using pulsed ultraviolet resonance Raman spectroscopy

H. Georg Schulze; L. Shane Greek; Michael W. Blades; Alan V. Bree; Boris B. Gorzalka; Robin F. B. Turner

doi:10.1117/12.273613

2 May 1997 Neurotransmitter measurement with a fiber optic probe using pulsed ultraviolet resonance Raman spectroscopy

H. Georg Schulze, L. Shane Greek, Michael W. Blades, Alan V. Bree, Boris B. Gorzalka, Robin F. B. Turner

Proceedings Volume 2982, Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology; (1997) https://doi.org/10.1117/12.273613
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

Many techniques have been developed to investigate the chemistry associated with brain activity. These techniques generally fall into two categories: fast techniques with species restricted sensitivity and slow techniques with generally unrestricted species sensitivity. Therefore, a need exists for a fast non-invasive technique sensitive to a wide array of biologically relevant compounds in order to measure chemical brain events in real time. The work presented here describes the progress made toward the development of a novel neurotransmitter probe. A fiber-optic linked Raman and tunable ultraviolet resonance Raman system was assembled with custom designed optical fiber probes. Probes of several different geometries were constructed and their working curves obtained in aqueous mixtures of methyl orange and potassium nitrate to determine the best probe configuration given particular sample characteristics. Using this system, the ultraviolet resonance Raman spectra of some neurotransmitters were measured with a fiber-optic probe and are reported here for the first time. The probe has also been used to measure neurotransmitter secretions obtained from depolarized rat pheochromocytoma cells.

Citation Download Citation

H. Georg Schulze, L. Shane Greek, Michael W. Blades, Alan V. Bree, Boris B. Gorzalka, and Robin F. B. Turner "Neurotransmitter measurement with a fiber optic probe using pulsed ultraviolet resonance Raman spectroscopy", Proc. SPIE 2982, Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology, (2 May 1997); https://doi.org/10.1117/12.273613

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