In-situ investigation of protein and DNA structure using UVRRS

L. Shane Greek; H. Georg Schulze; Michael W. Blades; Charles A. Haynes; Robin F. B. Turner

doi:10.1117/12.273609

2 May 1997 In-situ investigation of protein and DNA structure using UVRRS

L. Shane Greek, H. Georg Schulze, Michael W. Blades, Charles A. Haynes, Robin F. B. Turner

Proceedings Volume 2982, Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology; (1997) https://doi.org/10.1117/12.273609
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

Ultraviolet resonance Raman spectroscopy (UVRRS) has the potential to become a sensitive, specific, versatile bioanalytical and biophysical technique for routine investigations of proteins, DNA, and their monomeric components, as well as a variety smaller, physiologically important aromatic molecules. The transition of UVRRS from a complex, specialized spectroscopic method to a common laboratory assay depends upon several developments, including a robust sample introduction method permitting routine, in situ analysis in standard laboratory environments. To this end, we recently reported the first fiber-optic probes suitable for deep-UV pulsed laser UVRRS. In this paper, we extend this work by demonstrating the applicability of such probes to studies of biochemical relevance, including investigations of the resonance enhancement of phosphotyrosine, thermal denaturation of RNase T1, and specific and non-specific protein binding. The advantages and disadvantages of the probes are discussed with reference to sample conditions and probe design considerations.

Citation Download Citation

L. Shane Greek, H. Georg Schulze, Michael W. Blades, Charles A. Haynes, and Robin F. B. Turner "In-situ investigation of protein and DNA structure using UVRRS", Proc. SPIE 2982, Optical Diagnostics of Biological Fluids and Advanced Techniques in Analytical Cytology, (2 May 1997); https://doi.org/10.1117/12.273609

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