Data analysis in time-resolved fluorescence spectroscopy using computer simulation

Vladimir V. Apanasovich; Eugene G. Novikov; Nikolay N. Yatskov

doi:10.1117/12.273558

7 May 1997 Data analysis in time-resolved fluorescence spectroscopy using computer simulation

Vladimir V. Apanasovich, Eugene G. Novikov, Nikolay N. Yatskov

Proceedings Volume 2980, Advances in Fluorescence Sensing Technology III; (1997) https://doi.org/10.1117/12.273558
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

The detailed analysis of the processes of electronic energy relaxation in a substance, taking place after the short optical impulses of excitation, shows that mathematical models of the detected optical intensity decay can not be expressed by a combination of elementary mathematical functions. In the present work we suggest the approach to the parameter estimation of the decay curves, obtained from the time-resolved fluorescence experiments, based on the computer simulation methods. By means of simulation elementary processes of energy relaxation can be reproduced with the highest level of the detailed elaboration. Given processes are characterized by a set of parameters which have to be estimated. For the estimation we tried several methods, which do not require calculation of the derivatives, as in widespread Marquardt algorithm, that could be difficult for the decay curves, represented by a simulation model. The main advantage of the proposed approach is that it is possible to estimate the parameters of the physical processes without knowing their functional form. The series of simulation experiments on an estimation of parameters fluorescence decay kinetics confirms high potential of the considered approach.

Citation Download Citation

Vladimir V. Apanasovich, Eugene G. Novikov, and Nikolay N. Yatskov "Data analysis in time-resolved fluorescence spectroscopy using computer simulation", Proc. SPIE 2980, Advances in Fluorescence Sensing Technology III, (7 May 1997); https://doi.org/10.1117/12.273558

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