Alstonine as a potential fluorescent marker for tiny tumor detection and imaging

Pierre M. Viallet; Tuan Vo-Dinh; Jean-Marie Salmon; Wendi Watts; Emmanuelle Rocchi; Narayana R. Isola; Xavier Rebillard

doi:10.1117/12.275541

16 June 1997 Alstonine as a potential fluorescent marker for tiny tumor detection and imaging

Pierre M. Viallet, Tuan Vo-Dinh, Jean-Marie Salmon, Wendi Watts, Emmanuelle Rocchi, Narayana R. Isola, Xavier Rebillard

Proceedings Volume 2976, Biomedical Sensing, Imaging, and Tracking Technologies II; (1997) https://doi.org/10.1117/12.275541
Event: BiOS '97, Part of Photonics West, 1997, San Jose, CA, United States

Abstract

3,4,5,6,16,17-Hexadehydro-16-(methoxycarbolyl)-19(alpha) - methyl-20(alpha) -oxyohimbanium (alstonine) is a fluorescent alcaloid which is known to stain tumor cells more efficiently than normal. The interactions between alstonine and biological macromolecules were first investigated to provide the rationale for preferential labelling. Molecular filtration and spectrosfluorometric techniques with different macromolecules and isopolynucleotides have demonstrated that binding occurs only in the presence of uridyl rings. For the binding affect only the fluorescence intensity of alstonine it can be assumed that it involves only the side chain of the fluorescent compound. The capability for preferential staining was verified in culture using SK-OV-3 cells and rat hepatocarcinoma cells as tumor cells and Mouse fibroblasts or rat liver cells as controls. Techniques of image analysis have demonstrated the efficiency of cellular labelling even in aggregates of rat hepatocarcinoma. These experiments lead the way to the detection of tiny tumors developed on thin visceral walls, using a fiber optic device.

Citation Download Citation

Pierre M. Viallet, Tuan Vo-Dinh, Jean-Marie Salmon, Wendi Watts, Emmanuelle Rocchi, Narayana R. Isola, and Xavier Rebillard "Alstonine as a potential fluorescent marker for tiny tumor detection and imaging", Proc. SPIE 2976, Biomedical Sensing, Imaging, and Tracking Technologies II, (16 June 1997); https://doi.org/10.1117/12.275541

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KEYWORDS

Luminescence

Tumors

Liver

Fluorescent markers

Data acquisition

Imaging systems

Macromolecules

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