Absorption spectra reconstruction using Voigt profiles

R. Aguirre-Gomez; Alison R. Weeks; Simon R. Boxall

doi:10.1117/12.266466

6 February 1997 Absorption spectra reconstruction using Voigt profiles

R. Aguirre-Gomez, Alison R. Weeks, Simon R. Boxall

Proceedings Volume 2963, Ocean Optics XIII; (1997) https://doi.org/10.1117/12.266466
Event: Ocean Optics XIII, 1996, Halifax, Nova Scotia, Canada

Abstract

The identification of the components of algal absorption spectra can be made mathematically through either derivative or spectral deconvolution analyses. This work establishes a methodology for identifying phytoplankton pigments from absorption spectra by combining the derivative and spectral deconvolution methods and interpreting the results with the knowledge of the major light absorbing pigments. This was achieved a) by locating the position of the absorption peaks present in the spectra of different algal groups through the derivative analysis, b) by fitting a combination of Gaussian-Lorentzian or Voigt shaped bands, centered at these peaks, to the original spectra and c) by using pigment analysis via HPLC to provide the biological background for identifying these bands and peaks. The results of this study show that the derivative method can be used successfully in monocultures and natural populations to identify pigments by their individual absorption peaks. Secondly, a good agreement has been found between the original spectra and the reconstructed spectra which were a Voigt combination. The HPLC analysis provided the objective platform for the biological interpretation of the bands.

Citation Download Citation

R. Aguirre-Gomez, Alison R. Weeks, and Simon R. Boxall "Absorption spectra reconstruction using Voigt profiles", Proc. SPIE 2963, Ocean Optics XIII, (6 February 1997); https://doi.org/10.1117/12.266466

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