Optical characterization of a new photodiode array ocular fluorometer

Jose P.P. Domingues; Francisco A.M. Pinto; Carlos M.B.A. Correia; Jose G. Cunha-Vaz

doi:10.1117/12.260635

11 December 1996 Optical characterization of a new photodiode array ocular fluorometer

Jose P.P. Domingues, Francisco A.M. Pinto, Carlos M.B.A. Correia, Jose G. Cunha-Vaz

Proceedings Volume 2927, Optical and Imaging Techniques for Biomonitoring II; (1996) https://doi.org/10.1117/12.260635
Event: BiOS Europe '96, 1996, Vienna, Austria

Abstract

Ocular fluorometry has been considered an important technique in ophthalmology diagnosis and research. It consists in the application of a fluorometric method for determination of some important physiologic parameters in the eye in a non-invasive way. The need for new instrumentation for ocular fluorometry has been reported in the literature -- there is an ocular fluorometer commercially available, the Fluorotron Master, FM, but lack of axial resolution and high cost call for new instruments based on modern solid state sensors. Also the field of application of this technique is broadening due to availability of new tracers and exploitation of native fluorescence of ocular tissues and fluids (for which the FM is not fitted). In this paper we present the optics of a new instrument (ocular fluorometer) and report most of the tests to completely characterize the optical parameters of this fluorescence imaging system. Optical amplification, image spatial energy distribution over the detector -- a linear array of photodiodes, quantification and correction of slit- lamp angle, excitation and emission filter characteristics are some of the parameters that must be evaluated for a reliable interpretation of results. With a complete fluorometer characterization both in terms of performance parameters related mainly with the detector (linearity, sensitivity, reproducibility) in vitro and in vivo and in terms of optical system regarding the properties of the image obtained and possible aberrations correction by software we can perform reliable clinical evaluations of normal or abnormal situations in order to give the physician valid data he can store and recall for future reference.

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Jose P.P. Domingues, Francisco A.M. Pinto, Carlos M.B.A. Correia, and Jose G. Cunha-Vaz "Optical characterization of a new photodiode array ocular fluorometer", Proc. SPIE 2927, Optical and Imaging Techniques for Biomonitoring II, (11 December 1996); https://doi.org/10.1117/12.260635

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KEYWORDS

Sensors

Photodiodes

Luminescence

Fluorometers

Digital signal processing

Signal processing

Tissue optics

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