Autofluorescence properties of normal and transformed fibroblasts

Anna Cleta Croce; Alessandra Spano; Piera Balzarini; Donata Locatelli; Sergio Barni; Proto Pippia; Giovanni F. Bottiroli

doi:10.1117/12.260801

6 December 1996 Autofluorescence properties of normal and transformed fibroblasts

Anna Cleta Croce, Alessandra Spano, Piera Balzarini, Donata Locatelli, Sergio Barni, Proto Pippia, Giovanni F. Bottiroli

Author Affiliations +

Proceedings Volume 2926, Optical Biopsies and Microscopic Techniques; (1996) https://doi.org/10.1117/12.260801
Event: BiOS Europe '96, 1996, Vienna, Austria

Abstract

The autofluorescence properties of single living cells were studied through microspectrofluorometric analysis under different biological conditions. A tumor cell line, derived from a spontaneous sarcoma of a Galliera strain rat (SGS/3A), and normal fibroblasts (FG) at different culture passage numbers, derived from syngeneic rats, were used as a model. Under excitation at 366 nm autofluorescence is mainly due to the presence of coenzymes: NAD(P)H in the 440 - 480 nm spectral region, and flavines and lipopigments at wavelengths longer than 510 nm. Differences in the fluorescence emission amplitude and spectral shape are found in dependence on cell metabolic activity and transformation conditions.

Citation Download Citation

Anna Cleta Croce, Alessandra Spano, Piera Balzarini, Donata Locatelli, Sergio Barni, Proto Pippia, and Giovanni F. Bottiroli "Autofluorescence properties of normal and transformed fibroblasts", Proc. SPIE 2926, Optical Biopsies and Microscopic Techniques, (6 December 1996); https://doi.org/10.1117/12.260801

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available