New capillary optical fiber structure for fluorescence sensors

Pauli Kiiveri; Ari Petteri Hokkanen; Raija Kylmanen; Kari Keinanen; Simo K. T. Tammela

doi:10.1117/12.229944

10 January 1996 New capillary optical fiber structure for fluorescence sensors

Pauli Kiiveri, Ari Petteri Hokkanen, Raija Kylmanen, Kari Keinanen, Simo K. T. Tammela

Proceedings Volume 2695, Functional Photonic and Fiber Devices; (1996) https://doi.org/10.1117/12.229944
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

A multilayer capillary fiber was designed for optical sensor applications and its optical properties were evaluated by using a fluorescent layer immobilized on its inner surface. This fiber structure combines a large interaction surface (the inner wall of the capillary fiber) for binding of fluorescent indicators with evanescent wave fluorescence measurement, which may facilitate the design of pseudohomogeneous assays without separation of the bound from nonbound fluorescent indicator. The inner surface of the capillary was derivatized by aminosilanization, followed by biotinylation and addition of streptavidin. This biotin- streptavidin coating facilities subsequent immobilization of any biotinylated species (e.g. antibodies, antigens, etc.) participating in specific molecular recognition. We have evaluated some properties of this capillary fiber design by using fluorescent proteins immobilized on the inner wall of capillary by biotin-avidin-interaction. Fluorescence was excited by a HeNe-laser (fluorescent indicator APC; (lambda) _em equals 660 nm) and by a Ar-laser (fluorescent indicator RPE, (lambda) _em equals 578 nm), and measured with a spectrum analyzer.

Citation Download Citation

Pauli Kiiveri, Ari Petteri Hokkanen, Raija Kylmanen, Kari Keinanen, and Simo K. T. Tammela "New capillary optical fiber structure for fluorescence sensors", Proc. SPIE 2695, Functional Photonic and Fiber Devices, (10 January 1996); https://doi.org/10.1117/12.229944

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