Fiber optic light-scattering measurement system for evaluation of embryo viability: model experiment

Harumi Itoh; Tsunenori Arai; Makoto Kikuchi

doi:10.1117/12.239586

7 May 1996 Fiber optic light-scattering measurement system for evaluation of embryo viability: model experiment

Harumi Itoh, Tsunenori Arai, Makoto Kikuchi

Proceedings Volume 2681, Laser-Tissue Interaction VII; (1996) https://doi.org/10.1117/12.239586
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

We evaluated the particle density detectability and particle size detectivity of our fiber-optic light-scattering measurement system. In order to prevent the multiple pregnancy on current in vitro fertilization-embryo transfer, we have aimed to develop a new quantitative and non- invasive method to select a single viable human embryo. We employed the measurement of mitochondria localization in an embryo, which may have the correlation with development ability. We applied the angular distribution measurement of the light-scattering intensity from the embryo to obtain the information originated from the mitochondria. The latex spheres with a diameter of 1.0 micrometers were used to simulate the scattering intensity of the mitochondria. The measurement probes of our system consisted of two fibers for illumination and sensing. They were arranged at a right angle to a microscope optical axis to measure the angular distribution of the light-scattering intensity. We observed that the light-scattering intensity increased monotonically in the range from 10⁶ to 10¹⁰ particles per ml. Since the mitochondria density in a human embryo corresponded to 2.5 X 10⁷ per ml in the measurement chamber, we may measure the mitochondria density in the human embryo. The angular dependence of light-scattering intensity changed with the sphere diameters. This result showed the possibility of the selective measurement of the mitochondria density in the embryo in spite of the presence of the other cell organelle. We think that our light-scattering measurement system might be applicable to the evaluation method for the embryo viability.

Citation Download Citation

Harumi Itoh, Tsunenori Arai, and Makoto Kikuchi "Fiber optic light-scattering measurement system for evaluation of embryo viability: model experiment", Proc. SPIE 2681, Laser-Tissue Interaction VII, (7 May 1996); https://doi.org/10.1117/12.239586

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
5 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Scattering

Particles

Light scattering

Optical spheres

Latex

Laser scattering

Fiber optics

Show All Keywords

Keywords/Phrases

Search In:

Publication Years