Instrument factors influencing the precision of measurement of         clinical spectrometers

Gerald E. Cohn

doi:10.1117/12.237640

1 April 1996 Instrument factors influencing the precision of measurement of clinical spectrophotometers

Gerald E. Cohn

Proceedings Volume 2680, Ultrasensitive Biochemical Diagnostics; (1996) https://doi.org/10.1117/12.237640
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

Analytical instrument systems present assemblies of components which each introduces its own contribution to the overall system precision. Combining realistic models of detector response with a Beer's Law model for absorbance (A) provides a prediction for the overall system uncertainty. For the constant detector uncertainty model, reasonable values of sample parameters lead to substantial increases in the absorbance at minimum relative concentration error (RCE), the value of the RCE and the limits of the range of A values for best RCE. Similar effects follow from the square root detector model. In both detector models these effects are increased by concentration uncertainty. Assay chemistry factors dominate the system RCE at low A, making high precision difficult at low A.

Citation Download Citation

Gerald E. Cohn "Instrument factors influencing the precision of measurement of clinical spectrophotometers", Proc. SPIE 2680, Ultrasensitive Biochemical Diagnostics, (1 April 1996); https://doi.org/10.1117/12.237640

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