Holographic diagnostics of biological microparticles

Victor V. Dyomin; Vladimir V. Sokolov

doi:10.1117/12.239544

10 May 1996 Holographic diagnostics of biological microparticles

Victor V. Dyomin, Vladimir V. Sokolov

Proceedings Volume 2678, Optical Diagnostics of Living Cells and Biofluids; (1996) https://doi.org/10.1117/12.239544
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

Problem of studies of biological micro-ojects is actual one for ecology, medicine, biology. Holographic techniques are useful to solve the problem. The above microojects are transparent or semitransparent ones in a visible light rather often. The case of an optically soft particle, (that is of a particle whose substance has the refractive index close to that of the surrounding medium) is quite probable in biological water suspensions. Some peculiarities of holographing optically soft microparticles are analyzed in this paper. We propose a technique to calculate a light intensity distribution in the plane of a hologram and in the plane of a holographic image of a particle of an arbitrary shape at an arbitrary distance from the latter plane. The efficiency of the approach proposed is demonstrated by calculational results obtained analytically for some simple cases. In a more complicated cases the technique can make a basis for numerical computations. The method of determining of refractive index of transparent and semitransparent microparticles is proposed. We also present in this paper some experimental results on holographic detection of the water drops and such optically soft particles as ovums of helmints in human jaundice.

Citation Download Citation

Victor V. Dyomin and Vladimir V. Sokolov "Holographic diagnostics of biological microparticles", Proc. SPIE 2678, Optical Diagnostics of Living Cells and Biofluids, (10 May 1996); https://doi.org/10.1117/12.239544

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