Effects of indocyanine green in treatment of murine mammary tumor by an 808-nm diode laser: an in-vivo study

Wei R. Chen; Kelly G. Wichert; Aaron K. Higgins; Kenneth Eugene Bartels D.V.M.; Robert L. Adams; Robert E. Nordquist

doi:10.1117/12.237527

2 April 1996 Effects of indocyanine green in treatment of murine mammary tumor by an 808-nm diode laser: an in-vivo study

Wei R. Chen, Kelly G. Wichert, Aaron K. Higgins, Kenneth Eugene Bartels D.V.M., Robert L. Adams, Robert E. Nordquist

Author Affiliations +

Proceedings Volume 2675, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy V; (1996) https://doi.org/10.1117/12.237527
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

Indocyanine green was used to enhance laser-induced photothermal destruction of murine mammary tumor cells. The 808-nm diode laser used in these experiments matches the absorption peak of the indocyanine green. The combination of the laser and in situ administration of aqueous ICG provided a highly selective photothermal destruction pattern of the tumor tissue. Histology showed that within the power range of 3 to 5 watts. The ICG- targeted tumor tissues were fatally injured, while the peripheral tissues such as skin and other interdicting tissue not containing ICG were spared. Higher powers (10 to 15 watts) could inflict severe surface damage but only resulted in limited tissue penetration. Post-treatment observation also revealed surviving tumor cells, the cause of which might be the non-uniform distribution of ICG as well as the random scattering of photons inside tissue. After laser-ICG treatment, the tumor continued to grow, but at a slower rate, and to metastasize, leading to the death of the rats. The findings of our experiments question the long-term efficacy of the photothermal effect of a single treatment using the ICG and diode laser. However, the controlled killing of tumor cells on a large scale may be proven crucial when the treatment is applied repeatedly and/or in an earlier stage so that tumor growth could be stopped and metastases prevented. This photothermal interaction may also be effective when used in conjunction with other modalities.

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Wei R. Chen, Kelly G. Wichert, Aaron K. Higgins, Kenneth Eugene Bartels D.V.M., Robert L. Adams, and Robert E. Nordquist "Effects of indocyanine green in treatment of murine mammary tumor by an 808-nm diode laser: an in-vivo study", Proc. SPIE 2675, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy V, (2 April 1996); https://doi.org/10.1117/12.237527

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