Diffusion and localization of hematoporphyrin derivative in the normal bladder wall of a pig and a rat after local administration

Jean Francois Bisson; Dominique Notter; P. Labrude; C. Vigneron; Francois H. Guillemin

doi:10.1117/12.237534

2 April 1996 Diffusion and localization of hematoporphyrin derivative in the normal bladder wall of a pig and a rat after local administration

Jean Francois Bisson, Dominique Notter, P. Labrude, C. Vigneron, Francois H. Guillemin

Author Affiliations +

Proceedings Volume 2675, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy V; (1996) https://doi.org/10.1117/12.237534
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

Photochemotherapy using I.V. administered porphyrin photosensitizers has been used to treat superficial bladder cancers. In order to avoid cutaneous photosensitivity, lasting 6 - 8 weeks, we instilled the photosensitizer intravesically. After first studying the diffusion and localization of HpD in aqueous phase (5 mg/ml) in vitro through the bladder wall of pig by spectrofluorimetry ((lambda) _ex equals 392 nm and (lambda) _em equals 612.8 nm) and fluorescence microscopy, we determined the biodistribution of HpD in vivo in the rat bladder wall, 2 and 4 hours after bladder instillation of 0.4 ml of HpD: (1) the controls show only a weak autofluorescence restricted to the urothelium after 2 hours (24 micrometers plus or minus 5 micrometers, n equals 3) as well as after 4 hours (29.5 micrometers plus or minus 5 micrometers, n equals 3); (2) on the test preparation a higher fluorescence was observed: after 2 hours, HpD was localized in the urothelium and a very small part of the chorion (55 micrometers plus or minus 9 micrometers, n equals 9) whereas after 4 hours, it penetrated almost completely in the bladder wall (960 micrometers plus or minus 118 micrometers, n equals 9). In conclusion, a bladder instillation of 2 hours seems to be the optimal time of application in the rat since superficial bladder cancers, like carcinoma in situ, particularly occur in the urothelium (stage 0, pTa) or in the chorion (stage 1, pT1).

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Jean Francois Bisson, Dominique Notter, P. Labrude, C. Vigneron, and Francois H. Guillemin "Diffusion and localization of hematoporphyrin derivative in the normal bladder wall of a pig and a rat after local administration", Proc. SPIE 2675, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy V, (2 April 1996); https://doi.org/10.1117/12.237534

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