In-vivo immunofluorescence confocal microscopy of herpes simplex virus type 1 keratitis

Stephen C. Kaufman M.D.; Jeffery A. Laird; Roger W. Beuerman

doi:10.1117/12.240044

17 May 1996 In-vivo immunofluorescence confocal microscopy of herpes simplex virus type 1 keratitis

Stephen C. Kaufman M.D., Jeffery A. Laird, Roger W. Beuerman

Proceedings Volume 2673, Ophthalmic Technologies VI; (1996) https://doi.org/10.1117/12.240044
Event: Photonics West '96, 1996, San Jose, CA, United States

Abstract

The white-light confocal microscope offers an in vivo, cellular-level resolution view of the cornea. This instrument has proven to be a valuable research and diagnostic tool for the study of infectious keratitis. In this study, we investigate the direct visualization of herpes simplex virus type 1 (HSV-1)-infected corneal epithelium, with in vivo confocal microscopy, using HSV-1 immunofluorescent antibodies. New Zealand white rabbits were infected with McKrae strain of HSV-1 in one eye; the other eye of each rabbit was used as an uninfected control. Four days later, the rabbits were anesthetized and a cellulose sponge was applied to each cornea, and a drop of direct HSV fluorescein-tagged antibody was placed on each sponge every 3 to 5 minutes for 1 hour. Fluorescence confocal microscopy was then performed. The HSV-infected corneas showed broad regions of hyperfluorescent epithelial cells. The uninfected corneas revealed no background fluorescence. Thus, using the confocal microscope with a fluorescent cube, we were able to visualize HSV-infected corneal epithelial cells tagged with a direct fluorescent antibody. This process may prove to be a useful clinical tool for the in vivo diagnosis of HSV keratitis.

Citation Download Citation

Stephen C. Kaufman M.D., Jeffery A. Laird, and Roger W. Beuerman "In-vivo immunofluorescence confocal microscopy of herpes simplex virus type 1 keratitis", Proc. SPIE 2673, Ophthalmic Technologies VI, (17 May 1996); https://doi.org/10.1117/12.240044

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KEYWORDS

Confocal microscopy

Cornea

Microscopes

RGB color model

In vivo imaging

Luminescence

Eye

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