Three-dimensional computed tomography using a double-axis fluorescence microscope

Susumu Kikuchi; Kazuo Sonobe; Hidetoshi Yamada; Nagaaki Ohyama

doi:10.1117/12.237468

10 April 1996 Three-dimensional computed tomography using a double-axis fluorescence microscope

Susumu Kikuchi, Kazuo Sonobe, Hidetoshi Yamada, Nagaaki Ohyama

Proceedings Volume 2655, Three-Dimensional Microscopy: Image Acquisition and Processing III; (1996) https://doi.org/10.1117/12.237468
Event: Electronic Imaging: Science and Technology, 1996, San Jose, CA, United States

Abstract

We developed a double-axis fluorescence microscope as a new type of 3D microscope, in which two conventional microscopes are placed perpendicularly to one another. This optical microscope provides a solution to extend the spatial frequency band in the depth direction, which is greatly limited for conventional single-axis microscopes. In addition, this equipment has a capability of implementing a fast image acquisition system by simultaneously taking images through both microscopes. We evaluated the 3D imaging properties of our system using 3D observation images of a micro-fluorescent sphere. In order to reconstruct 3D images of micro-objects, we applied a CT-based method we proposed to the 3D microscopic imaging system. Our experiments using a biological micro-specimen verified usefulness and effectiveness of our system.

Citation Download Citation

Susumu Kikuchi, Kazuo Sonobe, Hidetoshi Yamada, and Nagaaki Ohyama "Three-dimensional computed tomography using a double-axis fluorescence microscope", Proc. SPIE 2655, Three-Dimensional Microscopy: Image Acquisition and Processing III, (10 April 1996); https://doi.org/10.1117/12.237468

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