Effects of the 633-nm laser on the behavior and morphology of primary fibroblast culture

Josepa Rigau; Chung-Ho Sun; Mario A. Trelles; Michael W. Berns

doi:10.1117/12.230027

15 January 1996 Effects of the 633-nm laser on the behavior and morphology of primary fibroblast culture

Josepa Rigau, Chung-Ho Sun, Mario A. Trelles, Michael W. Berns

Proceedings Volume 2630, Effects of Low-Power Light on Biological Systems; (1996) https://doi.org/10.1117/12.230027
Event: BiOS Europe '95, 1995, Barcelona, Spain

Abstract

We previously described the influence of low-level laser therapy (LLLT) on the primary fibroblast ATCC CRL1471 CCD-19SK passage 7 in culture, metabolic changes and statistical significance absorption of ³[H]Hydroxyproline after 2 irradiations (12 hour intervals) with Ar:DYE Laser, 633 nm wavelength, output power 38 mw, spot size 3.5 cm, power density 4 mw/cm², energy density plus or minus 2 J/cm². The aim of this work is to investigate, by using the same procedure, the behavior of the confluence monolayer fibroblasts culture when a central scratch of 0.4 - 1 mm and 2 irradiations is performed, by means of the study of the colony formation, haptotaxis (direction) and chemotaxis- chemokinesis (movement). The results indicate that all these phenomena appear sooner in the LLLT cultures than in non-treated cultures. Thus we can confirm that LLLT induces fibroblast biological effects.

Citation Download Citation

Josepa Rigau, Chung-Ho Sun, Mario A. Trelles, and Michael W. Berns "Effects of the 633-nm laser on the behavior and morphology of primary fibroblast culture", Proc. SPIE 2630, Effects of Low-Power Light on Biological Systems, (15 January 1996); https://doi.org/10.1117/12.230027

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available