New optical method for investigation of kinetics of nonspecific transmembrane transport into erythrocytes

Michail V. Fok; Galina A. Zaritskaya; Aleksandr R. Zaritskii; Elena V. Perevedentseva

doi:10.1117/12.229514

8 January 1996 New optical method for investigation of kinetics of nonspecific transmembrane transport into erythrocytes

Michail V. Fok, Galina A. Zaritskaya, Aleksandr R. Zaritskii, Elena V. Perevedentseva

Author Affiliations +

Proceedings Volume 2629, Biomedical Optoelectronics in Clinical Chemistry and Biotechnology; (1996) https://doi.org/10.1117/12.229514
Event: BiOS Europe '95, 1995, Barcelona, Spain

Abstract

A new optical method based on measurements of kinetics of the oxygenation and deoxygenation of erythrocytes in various functional states is developed. The new optic device, modeling the conditions of the system of circulation of the blood in organisms and allowing us to measure continuously the absolute values of degree of blood saturation by oxygen in the process of oxygenation (deoxygenation) and change of the mean erythrocyte volume, was elaborated. The analysis of obtained kinetic curves allowed us to determine the amount of hemoglobin capable to reverse association with oxygen (the active hemoglobin) in investigated sample and discover the pathology forms of hemoglobin. The new method allows us to investigate nonspecific erythrocyte membrane permeability. The rates of oxygen and glucose penetration to erythrocytes in various functional states was measured. It was discovered that erythrocyte membrane permeability for oxygen periodically considerably changes in the process of oxygenation and deoxygenation of the blood. It was found that the functional state of erythrocyte depends on the functional and structure state of erythrocyte membrane. The rate of glucose penetration to erythrocytes in various functional states was measured. The influence of external affects (physical and chemical, for example used in medicine surface-active substances) on function of the erythrocyte membrane was studied. Obtained results allow us to offer the elaborated device and methods of measurement as for scientific so for clinical investigations.

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Michail V. Fok, Galina A. Zaritskaya, Aleksandr R. Zaritskii, and Elena V. Perevedentseva "New optical method for investigation of kinetics of nonspecific transmembrane transport into erythrocytes", Proc. SPIE 2629, Biomedical Optoelectronics in Clinical Chemistry and Biotechnology, (8 January 1996); https://doi.org/10.1117/12.229514

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KEYWORDS

Blood

Oxygen

Lithium

Ions

Glucose

Chromium

Plasma

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