In-vitro investigations of the native fluorescence of lymph nodes

Wolfgang Lohmann; Thomas Dreyer; Rainer M. Bohle; Beate Pollich; Wolf-Bernhard Schill; Konrad Schwemmle

doi:10.1117/12.229979

15 January 1996 In-vitro investigations of the native fluorescence of lymph nodes

Wolfgang Lohmann, Thomas Dreyer, Rainer M. Bohle, Beate Pollich, Wolf-Bernhard Schill, Konrad Schwemmle

Proceedings Volume 2628, Optical and Imaging Techniques for Biomonitoring; (1996) https://doi.org/10.1117/12.229979
Event: BiOS Europe '95, 1995, Barcelona, Spain

Abstract

In in vitro illumination of HE stained frozen sections of healthy lymph nodes with different excitation wavelengths (365-546 nm) results, at least, in three different fluorescence emission peaks located at ca. 460, 545 and 592 nm. These might correspond to NADH, flavins, and protoporphyrin. Lymph nodes invaded by cancer cells resulted in the same spectra; they differ, however, in intensity. The HE stained cryosections can also be used for determining the spatial distribution of the endogenous fluorophores. In accordance with results obtained previously, cancer tissue of lymph nodes exhibit a small fluorescence intensity only. The adjacent healthy tissue shows a rather high intensity in comparison to normal values. Furthermore, necrotic tumor cells at different stages including its final degradation products (minute debris) can be detected by using a double illumination technique. HE stained frozen sections were illuminated concomitantly with a halogen lamp (bright-field transmitted light technique) and an Hg high-pressure lamp with an appropriate filter (395-440 nm) (incident light fluorescence technique). The minute debris can be seen in an area in which the histological image shows hardly anything. The fluorescence intensity observed in healthy tissue adjacent to the tumor, seems to decrease with progression of necrosis. Thus, autofluorescence studies on lymph nodes seem to be able to detect metastasis and the different stages of necrosis more clearly than the methods used conventionally.

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Wolfgang Lohmann, Thomas Dreyer, Rainer M. Bohle, Beate Pollich, Wolf-Bernhard Schill, and Konrad Schwemmle "In-vitro investigations of the native fluorescence of lymph nodes", Proc. SPIE 2628, Optical and Imaging Techniques for Biomonitoring, (15 January 1996); https://doi.org/10.1117/12.229979

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KEYWORDS

Luminescence

Lymphatic system

Tumors

Tissues

Cancer

Lamps

In vitro testing

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