Clinical laser-induced autofluorescence diagnosis of bladder tumors: dependence on the excitation wavelength

Dominique Ettori; Sigrid Avrillier; Maurice Anidjar M.D.; Oliver Cussenot; Alain Le Duc

doi:10.1117/12.228900

27 December 1995 Clinical laser-induced autofluorescence diagnosis of bladder tumors: dependence on the excitation wavelength

Dominique Ettori, Sigrid Avrillier, Maurice Anidjar M.D., Oliver Cussenot, Alain Le Duc

Author Affiliations +

Proceedings Volume 2627, Optical Biopsies; (1995) https://doi.org/10.1117/12.228900
Event: BiOS Europe '95, 1995, Barcelona, Spain

Abstract

The aim of this paper was to demonstrate the feasibility of routine real time clinical detection of occult urothelial precancerous and cancerous lesions by laser induced autofluorescence (LIAF), that is with no previous instillation of exogenous fluorescent marker. Three different pulsed laser wavelengths were alternately used for excitation: 480 nm (dye laser), 337 nm (nitrogen laser), and 308 nm (XeCl excimer laser). A clinical endoscopic study was performed on 23 patients immediately before transurethral resection of bladder tumor. Spectroscopic results were compared with histological analysis. For 480 nm and 337 nm excitation a single fluorescence broad band was obtained in any case, but for tumors the overall intensity was significantly reduced compared to normal mucosa. For 308 nm excitation, two main broad bands were observed. In the case of neoplasic lesions (including carcinoma in situ), the intensity ratio [I(360 nm)/I(440 nm)] was always greater than 2, but for normal or inflammatory areas this ratio was less than 2. A clear diagnosis could then be achieved for 308 nm excitation without the need of absolute intensity measurements. We get no false positive at this wavelength. XeCl LIAF spectroscopy is therefore a promising technique for the detection of urothelial precancerous lesions and could be used to perform an 'optical biopsy' in a routine mode with real time results.

Citation Download Citation

Dominique Ettori, Sigrid Avrillier, Maurice Anidjar M.D., Oliver Cussenot, and Alain Le Duc "Clinical laser-induced autofluorescence diagnosis of bladder tumors: dependence on the excitation wavelength", Proc. SPIE 2627, Optical Biopsies, (27 December 1995); https://doi.org/10.1117/12.228900

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