Near-field scanning optical microscope in biological research

Jia Wang; Dacheng Li

doi:10.1117/12.218688

6 September 1995 Near-field scanning optical microscope in biological research

Jia Wang, Dacheng Li

Proceedings Volume 2535, Near-Field Optics; (1995) https://doi.org/10.1117/12.218688
Event: SPIE's 1995 International Symposium on Optical Science, Engineering, and Instrumentation, 1995, San Diego, CA, United States

Abstract

Some problems in a near-field scanning optical microscope (NSOM) system for observing biological living specimens is discussed. The NSOM system works based on the principle of nano-optics and near-field optics. Propagating characteristics of evanescent wave field, the effects of absorption, polarization changes, fluorescence, optical density, refractive index, luminescence and incident angle are analyzed. Methods eliminating pseudo-image in specimens are proposed. NSOM will be employed to conduct investigation of single molecule of living biological specimen and make precision metrology researches in nanometer scale.

Citation Download Citation

Jia Wang and Dacheng Li "Near-field scanning optical microscope in biological research", Proc. SPIE 2535, Near-Field Optics, (6 September 1995); https://doi.org/10.1117/12.218688

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