Quantitative analysis of chromosome motion in Drosophila melanogaster

Wallace Frank Marshall; David A. Agard; John W. Sedat

doi:10.1117/12.205345

23 March 1995 Quantitative analysis of chromosome motion in Drosophila melanogaster

Wallace Frank Marshall, David A. Agard, John W. Sedat

Proceedings Volume 2412, Three-Dimensional Microscopy: Image Acquisition and Processing II; (1995) https://doi.org/10.1117/12.205345
Event: IS&T/SPIE's Symposium on Electronic Imaging: Science and Technology, 1995, San Jose, CA, United States

Abstract

We present an algorithm for estimating nonrigid motion of chromosomes in 4D microscopic images. Chromosomes are represented by a graph and motion estimation is formulated as a graph matching problem. All chromosomes within the graph are located, and then simulated annealing is used to find the mapping of chromosomes at time t onto chromosomes at time t+1 that minimizes the integrated displacement along each chromosome. Results with actual 4D images indicate that this model-based approach is sufficiently robust to correctly track the motion of chromosomes under conditions of limited spatial and temporal resolution. Using the motion estimate, previously developed methods for the quantitative analysis of 3D structure are extended to four dimensions, allowing chromosome mobility, flexibility, and interactions to be measured. Application of these algorithms to 4D images of Drosophila metaphase chromosomes in vivo allows visualization of clearly defined domains of high chromosomal flexibility, as well as other regions of distinctly lower chromosomal mobility which may coincide with centrometers.

Citation Download Citation

Wallace Frank Marshall, David A. Agard, and John W. Sedat "Quantitative analysis of chromosome motion in Drosophila melanogaster", Proc. SPIE 2412, Three-Dimensional Microscopy: Image Acquisition and Processing II, (23 March 1995); https://doi.org/10.1117/12.205345

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