Characterization of endogenous fluorophores by picosecond laser fluorescence spectroscopy

Wolfgang Hoehne; Werner Schramm; Mathias Nittka; Heinz Detlef Kronfeldt

doi:10.1117/12.198735

4 January 1995 Characterization of endogenous fluorophores by picosecond laser fluorescence spectroscopy

Wolfgang Hoehne, Werner Schramm, Mathias Nittka, Heinz Detlef Kronfeldt

Proceedings Volume 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging; (1995) https://doi.org/10.1117/12.198735
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France

Abstract

By time resolved fluorescence spectroscopy in the psec range the fluorescence behavior of flavines as important endogenous fluorophores was investigated. The substances were examined under various conditions (e.g. pure solutions and cellular suspensions; different buffer systems and pH values). Particular attention was dedicated to the properties of these coenzymes under in situ conditions. The results were applied to test numerical methods for characterization of complex mixtures of fluorophores and for discrimination of different states of flavines.

Citation Download Citation

Wolfgang Hoehne, Werner Schramm, Mathias Nittka, and Heinz Detlef Kronfeldt "Characterization of endogenous fluorophores by picosecond laser fluorescence spectroscopy", Proc. SPIE 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging, (4 January 1995); https://doi.org/10.1117/12.198735

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