Water molecules are active during the primary photoreaction of bacteriorhodopsin

Wolfgang B. Fischer; Kenneth J. Rothschild

doi:10.1117/12.166685

31 January 1994 Water molecules are active during the primary photoreaction of bacteriorhodopsin

Wolfgang B. Fischer, Kenneth J. Rothschild

Proceedings Volume 2089, 9th International Conference on Fourier Transform Spectroscopy; (1994) https://doi.org/10.1117/12.166685
Event: Fourier Transform Spectroscopy: Ninth International Conference, 1993, Calgary, Canada

Abstract

FT-IR difference spectra of the primary photoreaction of bacteriorhodopsin, the light driven proton pump from Halobacterium halobium, are presented with data above 3500 cm^-1. Samples were hydrated in H₂O, H₂¹⁸O and D₂O in order to distinguish vibrational bands due to water. In the bRyieldsK difference spectrum, evidence is found for a water molecule which undergoes a strengthening in hydrogen bonding. In contrast, during the KyieldsL and LyieldsM steps of the photocycle one or more additional water molecules undergo a weakening in hydrogen bonding.

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Wolfgang B. Fischer and Kenneth J. Rothschild "Water molecules are active during the primary photoreaction of bacteriorhodopsin", Proc. SPIE 2089, 9th International Conference on Fourier Transform Spectroscopy, (31 January 1994); https://doi.org/10.1117/12.166685

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