Structural characterization and nanometer-scale domain formation in phospholipid model membranes by infrared spectroscopy and scanning tunneling microscopy

Brian W. Gregory; Richard A. Dluhy; Lawrence A. Bottomley

doi:10.1117/12.166605

31 January 1994 Structural characterization and nanometer-scale domain formation in phospholipid model membranes by infrared spectroscopy and scanning tunneling microscopy

Brian W. Gregory, Richard A. Dluhy, Lawrence A. Bottomley

Author Affiliations +

Proceedings Volume 2089, 9th International Conference on Fourier Transform Spectroscopy; (1994) https://doi.org/10.1117/12.166605
Event: Fourier Transform Spectroscopy: Ninth International Conference, 1993, Calgary, Canada

Abstract

Research in this laboratory is presently being conducted towards the structural characterization of well-ordered molecular monolayers using an integrated spectroscopic/imaging approach. We are currently using infrared spectroscopy (IR) in combination with scanning tunneling microscopy (STM) on both Langmuir-Blodgett (L-B) and self-assembled (SA) monolayer films in order to obtain compositional, conformational, and topographic information about these films. We have constructed organized lipid bilayer assemblies using a combination of SA and L-B methods which were studied using this combined IR/STM approach. These studies showed acyl chain tilt angles of 5 - 10 degree(s) within the DPPG monolayer by IR and STM. In addition, nanometer-size DPPG with hexagonal-type grain boundaries, and molecular resolution of phosphoglycerol headgroup structure within these domains, have been observed by STM. Phospholipid headgroup areas are in excellent agreement with previous x-ray diffraction studies of this system.

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Brian W. Gregory, Richard A. Dluhy, and Lawrence A. Bottomley "Structural characterization and nanometer-scale domain formation in phospholipid model membranes by infrared spectroscopy and scanning tunneling microscopy", Proc. SPIE 2089, 9th International Conference on Fourier Transform Spectroscopy, (31 January 1994); https://doi.org/10.1117/12.166605

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