Production of electrostatically-charged biomolecules by induction charging of a liquid jet with Fourier transform infrared spectrometry detection

Vincent E. Turula; James A. de Haseth

doi:10.1117/12.166680

31 January 1994 Production of electrostatically charged biomolecules by induction charging of a liquid jet with Fourier transform infrared spectrometry detection

Vincent E. Turula, James A. de Haseth

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Proceedings Volume 2089, 9th International Conference on Fourier Transform Spectroscopy; (1994) https://doi.org/10.1117/12.166680
Event: Fourier Transform Spectroscopy: Ninth International Conference, 1993, Calgary, Canada

Abstract

The Particle Beam LC/FT-IR interface has been modified so that the aerosol produced can be electrostatically charged by the induction charging (IC) technique. Manipulation of the beam of uniformly charged particles, by electrodynamics using potential arrays, in addition to physical alignment, enhances analyte transport efficiency. The emphasis of this work involves FT-IR analysis of biomolecules prepared from an electrostatic aerosol. With IC, and potential influence over the trajectories, collection of the desolvated biomolecule can be achieved without the high-energy impact associated with normal particle beam operation, an ideal situation for conformation studies in which structure is often affected by the harsh collision. In this work the globular proteins, (alpha) -Chymotrypsin, Papain, and the amino acid d,l- Tryptophan were electrostatically charged by the IC technique, and deposited onto IR- transparent substrates which produced films that were examined by FT-IR.

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Vincent E. Turula and James A. de Haseth "Production of electrostatically charged biomolecules by induction charging of a liquid jet with Fourier transform infrared spectrometry detection", Proc. SPIE 2089, 9th International Conference on Fourier Transform Spectroscopy, (31 January 1994); https://doi.org/10.1117/12.166680

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KEYWORDS

Aerosols

Particle beams

FT-IR spectroscopy

Liquids

Electrodes

Spectroscopy

Proteins

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