Genosensors: microfabricated devices for automated DNA sequence analysis

Mitchell D. Eggers; Michael E. Hogan; Robert K. Reich; Jagannath B. Lamture; Ken Beattie; Mark A. Hollis; Daniel J. Ehrlich; Bernard B. Kosicki; John M. Shumaker; Raj S. Varma; Barry E. Burke; Al Murphy; Dennis D. Rathman

doi:10.1117/12.146711

24 June 1993 Genosensors: microfabricated devices for automated DNA sequence analysis

Mitchell D. Eggers, Michael E. Hogan, Robert K. Reich, Jagannath B. Lamture, Ken Beattie, Mark A. Hollis, Daniel J. Ehrlich, Bernard B. Kosicki, John M. Shumaker, Raj S. Varma, Barry E. Burke, Al Murphy, Dennis D. Rathman

Author Affiliations +

Proceedings Volume 1891, Advances in DNA Sequencing Technology; (1993) https://doi.org/10.1117/12.146711
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

A new technology is introduced for developing potentially low cost, high throughput DNA sequence analysis. This approach utilizes novel bioelectronic genosensor devices to rapidly detect hybridization events across a DNA probe array. Detection of DNA probe/target hybridization has been achieved by two electronic methods. The first method utilizes a permittivity chip which interrogates the miniature test fixtures with a low voltage alternating electric field. The second method, which is the emphasis of this paper, utilizes a charge- coupled device (CCD) to detect the hybridization of appropriately tagged (radioisotope, fluorescent, or chemiluminescent labels) target DNA to an array of DNA probes immobilized above the pixels. Such direct electronic-biologic coupling is shown to provide a tenfold sensitivity improvement over conventional lens-based detection systems.

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Mitchell D. Eggers, Michael E. Hogan, Robert K. Reich, Jagannath B. Lamture, Ken Beattie, Mark A. Hollis, Daniel J. Ehrlich, Bernard B. Kosicki, John M. Shumaker, Raj S. Varma, Barry E. Burke, Al Murphy, and Dennis D. Rathman "Genosensors: microfabricated devices for automated DNA sequence analysis", Proc. SPIE 1891, Advances in DNA Sequencing Technology, (24 June 1993); https://doi.org/10.1117/12.146711

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