Time-correlated photon counting: differential methods applied to anisotropy decay

Douglas Magde; Michael D. Magde

doi:10.1117/12.144727

18 May 1993 Time-correlated photon counting: differential methods applied to anisotropy decay

Douglas Magde, Michael D. Magde

Proceedings Volume 1885, Advances in Fluorescence Sensing Technology; (1993) https://doi.org/10.1117/12.144727
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

A method is described that makes it possible to measure luminescence decays for both parallel and perpendicular excitation geometries in a single experimental run. The rotational diffusion of oxazine 725 in five solvents of different viscosities is characterized using the new procedure. The measurements are challenging in that the rotational diffusion times are close to the excited state lifetime. The advantage of the new procedure lies in the increased confidence that comes with measuring both polarizations under the same conditions. The same principle is applicable in any circumstance involving the comparison of two or more samples.

Citation Download Citation

Douglas Magde and Michael D. Magde "Time-correlated photon counting: differential methods applied to anisotropy decay", Proc. SPIE 1885, Advances in Fluorescence Sensing Technology, (18 May 1993); https://doi.org/10.1117/12.144727

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available