Effect of photodynamic therapy on mouse platelets

Chuannong Zhou; Shunji Chi; Jinsheng Deng; Hua Zhang; Junlin Liang; Xian-wen Ha

doi:10.1117/12.146295

18 June 1993 Effect of photodynamic therapy on mouse platelets

Chuannong Zhou, Shunji Chi, Jinsheng Deng, Hua Zhang, Junlin Liang, Xian-wen Ha

Proceedings Volume 1881, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy II; (1993) https://doi.org/10.1117/12.146295
Event: OE/LASE'93: Optics, Electro-Optics, and Laser Applications in Scienceand Engineering, 1993, Los Angeles, CA, United States

Abstract

Normal mice received hematoporphyrin derivative (HpD) i.v. prior to red light irradiation and the platelet-rich plasma was prepared and irradiated by red light. The platelets were processed for EM examination and stereological analysis. It was shown the 16 hrs after irradiation almost all platelets were necrotized; 8 hours after irradiation about one fourth of the platelets were necrotized and the remaining were considerably damaged. Immediately after irradiation a small number of platelets became necrotic and most other platelets were swollen and deformated, showing significantly increased mean area, perimeter and short axis, and mean cell volume and cell surface area. The findings indicate that platelets are highly sensitive to PDT action and can be directly and rapidly damaged by PDT even in the absence of vascular endothelial cells. The early platelet photoactivation may play an important role in the initiation of early vascular damage and microcirculatory alterations induced by PDT in vivo.

Citation Download Citation

Chuannong Zhou, Shunji Chi, Jinsheng Deng, Hua Zhang, Junlin Liang, and Xian-wen Ha "Effect of photodynamic therapy on mouse platelets", Proc. SPIE 1881, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy II, (18 June 1993); https://doi.org/10.1117/12.146295

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