Phase-contrast microscopy in the in-vitro study of neoplastic cell dynamic morphology and behavior as related to malignancy

Pavel Vesely

doi:10.1117/12.171869

3 May 1994 Phase-contrast microscopy in the in-vitro study of neoplastic cell dynamic morphology and behavior as related to malignancy

Pavel Vesely

Author Affiliations +

Proceedings Volume 1846, Phase Contrast and Differential Interference Contrast Imaging Techniques and Applications; (1994) https://doi.org/10.1117/12.171869
Event: Phase Contrast and Differential Interference Contrast Imaging Techniques and Applications, 1992, Warsaw, Poland

Abstract

Phase contrast microscopy plays a major role in establishing knowledge on in vitro cell behavior in cultured cells. A major focus of these studies has been on the identification of differences between normal and neoplastic cells. Spontaneously metastasizing rat sarcoma was studied to determine the relationship between the rate of appearance of spontaneous metastases and the patterns of in vitro cell behavior. It was found that a slightly acid extracellular milieu (pH 6.5), simulating the natural situation within the tumor, brought about stimulation of directional migration in highly malignant phenotypes. That type of behavior may account for malignant spread of a neoplastic cell from the primary site of the tumor. A set of the in vitro observable properties of the malignant cell phenotype is detailed here and called activated morphotype. A working theory about the origin of the activated morphotype of the neoplastic cell (AMNC) is presented.

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Pavel Vesely "Phase-contrast microscopy in the in-vitro study of neoplastic cell dynamic morphology and behavior as related to malignancy", Proc. SPIE 1846, Phase Contrast and Differential Interference Contrast Imaging Techniques and Applications, (3 May 1994); https://doi.org/10.1117/12.171869

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