Investigation of vesicle-capsular plague antigen complex formation by elastic laser radiation scattering

N. P. Guseva; Irina S. Maximova; Sergey V. Romanov; L. P. Shubochkin; Sergey N. Tatarintsev

doi:10.1117/12.57274

1 May 1991 Investigation of vesicle-capsular plague antigen complex formation by elastic laser radiation scattering

N. P. Guseva, Irina S. Maximova, Sergey V. Romanov, L. P. Shubochkin, Sergey N. Tatarintsev

Proceedings Volume 1403, Laser Applications in Life Sciences; (1991) https://doi.org/10.1117/12.57274
Event: Laser Applications in Life Sciences, 1990, Moscow, Russian Federation

Abstract

Recently a great deal of attention has been given to the investigation artificial lipid liposomes, due to their application as "containers" for directed transport of biologically active compounds into particular cells, organs and tissues for prophylaxis and therapy of infectious diseases. The use of traditional methods of liposome investigation, such as sedimentation, electrophoresis and chromatography is impeded by low liposome resistivity to different deformations. In conjunction with this, optical methods of laser light scattering are promising as they allow nondisturbing, precise and quick investigations. This paper describes the investigation of vesicle systems prepared from egg lecithin of Serva Corporation and their complexes with the capsular antigen of the plague microbe. The capsular antigen Fl was obtained from EV plague microbe grown at 37° C on Huttinger agar. Fl was isolated by gel-filtration on ASA-22 followed by freeze drying of the preparation. Angular dependences of polarized radiation scattering were measured for several liposome suspension samples in a saline solution before and after the interaction with the plague microbe capsular antigen. The aim of the investigation was to analyze the nature of mutual antigen arrangement in a liposome and to develop methods for measuring its inclusion percentage.

Citation Download Citation

N. P. Guseva, Irina S. Maximova, Sergey V. Romanov, L. P. Shubochkin, and Sergey N. Tatarintsev "Investigation of vesicle-capsular plague antigen complex formation by elastic laser radiation scattering", Proc. SPIE 1403, Laser Applications in Life Sciences, (1 May 1991); https://doi.org/10.1117/12.57274

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