Distribution of fluorescent probe molecules throughout the phospholipid membrane depth

Nicolai A. Nemkovich; Anatoly Nikolaevich Rubinov; M. G. Savvidi; V. I. Tomin; Nina V. Shcherbatska

doi:10.1117/12.57387

1 May 1991 Distribution of fluorescent probe molecules throughout the phospholipid membrane depth

Nicolai A. Nemkovich, Anatoly Nikolaevich Rubinov, M. G. Savvidi, V. I. Tomin, Nina V. Shcherbatska

Proceedings Volume 1403, Laser Applications in Life Sciences; (1991) https://doi.org/10.1117/12.57387
Event: Laser Applications in Life Sciences, 1990, Moscow, Russian Federation

Abstract

A method to determine the distribution function shape of the luminescent probe molecules over biological membrane depth is suggested. It is based on: - the unhomogeneous model of the probe ensemble which permits its selection alteration selectively of the position and distribution of excited probe molecules by the wavelength of the excitation light - the use of the nonradiative electronic energy transfer (NEET) mechanism from probe molecules in vesicules to the acceptor ones in a buffer solution. The distribution function of the donor-excited molecules for different wavelength of the excitation light was calculated. Using the spectra of probe molecules at different Ae and Lippert''s equation the values of the dielectric constant of the membrane e were obtained for different regions. 1.

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Nicolai A. Nemkovich, Anatoly Nikolaevich Rubinov, M. G. Savvidi, V. I. Tomin, and Nina V. Shcherbatska "Distribution of fluorescent probe molecules throughout the phospholipid membrane depth", Proc. SPIE 1403, Laser Applications in Life Sciences, (1 May 1991); https://doi.org/10.1117/12.57387

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KEYWORDS

Molecules

Luminescence

Dielectrics

Absorption

Laser applications

Molecular energy transfer

Life sciences

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