Design of wide-band high numerical aperture flat field apochromatic microscope objective

Xinchun Zhang; Xinyu Liu; Jingsheng Wang; Jing Wu; Ziyu Zhu; Zhigao Sheng

doi:10.1117/12.3052701

18 November 2024 Design of wide-band high numerical aperture flat field apochromatic microscope objective

Xinchun Zhang, Xinyu Liu, Jingsheng Wang, Jing Wu, Ziyu Zhu, Zhigao Sheng

Author Affiliations +

Proceedings Volume 13417, International Conference on Precision Instruments and Optical Engineering (PIOE 2024); 1341708 (2024) https://doi.org/10.1117/12.3052701
Event: 4th International Conference on Precision Instruments and Optical Engineering (PIOE 2024), 2024, Kuala Lumpur, Malaysia

Abstract

In the field of microscopic imaging, such as gene detection of cancer cells and fluorescence microscopic imaging, wider spectral coverage, high numerical aperture and precise chromatic aberration correction are required for microscopic objectives. In this paper, optical software ZEMAX is used to design a flat-field apochromatic microscopic objective with a magnification of 40× and a numerical aperture of 0.8. The objective lens has the characteristics of wide spectral coverage, high numerical aperture and strict color correction. The results of design and analysis show that the flat field number of the objective is 1.15um and the maximum focal shift is less than 0.5um, which conforms to the international standard of microscopic objective. The modulation transfer function (MTF) approaches the diffraction limit at the frequency up to 208lp/mm, and the objective achieves a flat-field apochromatic design in the range of 400-1000nm. The tolerance analysis results show that the MTF value at 208lp/mm has an 80% probability of being better than 0.5974, and machining and assembly can be carried out according to this tolerance allocation.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Xinchun Zhang, Xinyu Liu, Jingsheng Wang, Jing Wu, Ziyu Zhu, and Zhigao Sheng "Design of wide-band high numerical aperture flat field apochromatic microscope objective", Proc. SPIE 13417, International Conference on Precision Instruments and Optical Engineering (PIOE 2024), 1341708 (18 November 2024); https://doi.org/10.1117/12.3052701

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KEYWORDS

Objectives

Design

Tolerancing

Microscopes

Modulation transfer functions

Monochromatic aberrations

Glasses

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