Spatial-frequency-domain hyperspectral microscopy

Lisa Uguen; Ronan Piedevache; Gaspard Russias; Sofian Helmer; Antoine Fournier; Denis Tregoat; Stéphane Perrin

doi:10.1117/12.3016738

18 June 2024 Spatial-frequency-domain hyperspectral microscopy

Lisa Uguen, Ronan Piedevache, Gaspard Russias, Sofian Helmer, Antoine Fournier, Denis Tregoat, Stéphane Perrin

Proceedings Volume 12996, Unconventional Optical Imaging IV; 129960S (2024) https://doi.org/10.1117/12.3016738
Event: SPIE Photonics Europe, 2024, Strasbourg, France

Conference Poster

Abstract

Hyperspectral imaging allows to reconstruct both spatial and quasi-continuous spectral information of an object (i.e., an hypercube), unlike multispectral imaging where the spectrum is divided in few bands. This work shows the innovating combination of single-pixel hyperspectral imaging with reflective-mode optical microscopy to retrieve high-spatial-resolution hypercubes of samples in the visible and in the near-infrared ranges. The spatial resolution reaches around 2.5 μm and the spectral information is sampled over around 200 spectral bands. Furthermore, statistical algorithms enables the clustering of regions of interest of inorganic and organic samples and the identification of spectral responses of targeted cells.

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Lisa Uguen, Ronan Piedevache, Gaspard Russias, Sofian Helmer, Antoine Fournier, Denis Tregoat, and Stéphane Perrin "Spatial-frequency-domain hyperspectral microscopy", Proc. SPIE 12996, Unconventional Optical Imaging IV, 129960S (18 June 2024); https://doi.org/10.1117/12.3016738

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