Caroline E. Filan,1,2 Srinidhi Bharadwaj,2 Paloma Casteleiro Costa,2,1 Amin Davarzani,3 Dan Cappabianca,4 Anna Tommasi,4 Lauren Sarko,4 Nina La Vonne Denne,4 Leidong Mao,3 Krishanu Saha,4 Lohitash Karumbaiah,3 Francisco E. Robleshttps://orcid.org/0000-0003-4413-97791,2
1Georgia Institute of Technology (United States) 2Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory Univ. (United States) 3The Univ. of Georgia (United States) 4Univ. of Wisconsin-Madison (United States)
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
Quantitative oblique back-illumination microscopy (qOBM) is a label-free imaging technique that enables tomographic phase imaging of thick scattering samples with epi-illumination. Here, we propose the use of two forms of functional imaging with qOBM to study tissue and cell cultures. In doing so, we obtain the spatiotemporal and quantitative functional information associated with the phase values extrapolated from qOBM imaging. We have applied this process to study the efficacy of individual immune T cells to kill glioblastoma spheroid cultures in 3D spheroids. Data show that we can effectively distinguish between cell phenotypes and characterize the dynamic motion of these cells in 3D cultures. This work offers a distinct advantage in tracking 3D cellular dynamics in thick tissue as many function imaging modalities are limited to 2D samples. Further, this technology can be expanded to analyze a wide variety of cellular and subcellular dynamics non-invasively in thick tissue.
(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.