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12 March 2024 Three-dimensional multi-directional light-sheet microscopy with structured illumination
Ming Song, Yang Liu, Hsueh-Fu Wu, Nadja Zeltner, Peter A. Kner
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Proceedings Volume 12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI; 1284809 (2024) https://doi.org/10.1117/12.3000528
Event: SPIE BiOS, 2024, San Francisco, California, United States
Abstract
Light-Sheet Fluorescence Microscopy (LSFM) has demonstrated its effectiveness in imaging many model organisms. However, traditional LSFMs often exhibit low resolution and encounter striping artifacts. Here, we introduce a novel LSFM design to address these challenges of imaging large biological samples. Our system employs a single-objective light-sheet geometry, incorporating Structured Illumination Microscopy (SIM) to allow multi-direction light-sheet illumination. This combination aims to rectify striping artifacts and achieve improved lateral resolutions of better than 200 nm over a 277 × 277 × 500 μm3 volume. Our system is demonstrated through imaging beads, C. elegans and cerebellum organoids.
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Ming Song, Yang Liu, Hsueh-Fu Wu, Nadja Zeltner, and Peter A. Kner "Three-dimensional multi-directional light-sheet microscopy with structured illumination", Proc. SPIE 12848, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI, 1284809 (12 March 2024); https://doi.org/10.1117/12.3000528
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KEYWORDS
Biological imaging
Biological samples
Imaging systems
Microscopy
Image resolution
Resolution enhancement technologies
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