Poster
13 March 2024 Interest of diffractive mask in a VIPA-based Brillouin spectrometer for biological sample
Alban Desoutter, Remy Vialla, Frédéric Cuisinier, Y. Messat, Hamideh Salehi, Emmanuel Rousseau, Benoit Rufflé
Author Affiliations +
Conference Poster
Abstract
The need to characterize the mechanical properties of cells led biologists to promote spectroscopy based on Brillouin scattering. This rapidly required a balance between two important criteria: on the one hand, the acquisition time for individual spectra, and on the other, sufficient contrast to observe low-intensity signals.

Spectrometers based on VIPA (Virtually imaged phased array) give the possibility of obtaining the full spectrum in just a few hundred milliseconds, with contrasts that make it possible to study biological samples.

The addition of a Lyot mask therefore makes it possible, at low cost, to suppress part of the background of the recorded signal, using the geometry of the pattern formed by the two Rayleigh peak suppression slits. This addition, if optimally placed, enables effective transmission to be maintained for Brillouin shift measurement, while gaining 25 dB in contrast.

Thus, the configuration presented here, combining a trick for reducing specular reflection at the sample/substrate interface and a device for obtaining measurements under good conditions, makes it possible to envisage combining this spectroscope with other spectroscopy.
(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.
Alban Desoutter, Remy Vialla, Frédéric Cuisinier, Y. Messat, Hamideh Salehi, Emmanuel Rousseau, and Benoit Rufflé "Interest of diffractive mask in a VIPA-based Brillouin spectrometer for biological sample", Proc. SPIE 12844, Optical Elastography and Tissue Biomechanics XI, 128440E (13 March 2024); https://doi.org/10.1117/12.3002903
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KEYWORDS
Spectroscopy

Biological samples

Cameras

Scattering

Spherical lenses

Elasticity

Fourier transforms

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