Improving the photodeactivation efficiency of pathogens using methylene blue with silver nanoparticles and efflux pump inhibitors

Yaran Allamyradov; Justice ben Yosef; Salizhan Kylychbekov; Zikrulloh Khuzhakulov; Inomjon Majidov; Simran Banga; Ali O. Er

doi:10.1117/12.3001067

12 March 2024 Improving the photodeactivation efficiency of pathogens using methylene blue with silver nanoparticles and efflux pump inhibitors

Yaran Allamyradov, Justice ben Yosef, Salizhan Kylychbekov, Zikrulloh Khuzhakulov, Inomjon Majidov, Simran Banga, Ali O. Er

Author Affiliations +

Proceedings Volume 12822, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2024; 1282209 (2024) https://doi.org/10.1117/12.3001067
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Bacterial antibiotic resistance poses a pressing global health crisis, challenging conventional therapies. Efflux pumps diminish antimicrobial effectiveness by expelling drugs from bacterial cells. Multidrug efflux pumps (MEPs) have been found to transport diverse compounds, including phenothiazinium dyes like methylene blue, out of bacteria. Inhibition of MEPs offers a promising strategy to bolster the efficacy of antimicrobial photodynamic inactivation (PDT). This research adopts a synergistic approach, combining the efflux pump inhibitor (EPI) , reserpine, with silver nanoparticles (Ag NPs) and methylene blue (MB) to enhance PDT efficiency. Ag NPs were synthesized via pulsed laser ablation and characterized using TEM, UV-Vis, and PL spectra. E. coli was treated with MB, Ag NPs, and reserpine, followed by LED light irradiation. MB was twice as effective, and AgNPs/MB was six times more effective with reserpine during a sixminute irradiation. Ongoing experiments on morphological changes will be presented. AgNPs/MB with reserpine could effectively combat bacterial pathogens in open wounds and prosthetic joint infections.

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(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Yaran Allamyradov, Justice ben Yosef, Salizhan Kylychbekov, Zikrulloh Khuzhakulov, Inomjon Majidov, Simran Banga, and Ali O. Er "Improving the photodeactivation efficiency of pathogens using methylene blue with silver nanoparticles and efflux pump inhibitors", Proc. SPIE 12822, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2024, 1282209 (12 March 2024); https://doi.org/10.1117/12.3001067

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