Highly temporal phase stable common-path quantitative phase microscope

Anuj Saxena; Azeem Ahmad; Vishesh Dubey; Sathi Das; Anowarul Habib; Balpreet Singh Ahluwalia; Dalip Singh Mehta

doi:10.1117/12.2671032

9 August 2023 Highly temporal phase stable common-path quantitative phase microscope

Anuj Saxena, Azeem Ahmad, Vishesh Dubey, Sathi Das, Anowarul Habib, Balpreet Singh Ahluwalia, Dalip Singh Mehta

Proceedings Volume 12630, Advances in Microscopic Imaging IV; 126300P (2023) https://doi.org/10.1117/12.2671032
Event: European Conferences on Biomedical Optics, 2023, Munich, Germany

Abstract

Quantitative phase microscope (QPM) is used for the quantitative information and dynamic phase imaging of biological specimen, which provides wide application in biomedical sciences. High temporal phase stability of the QPM system is the primary requirement for accurate phase measurement. We have developed a common-path QPM geometry based on beam displacer and pinhole unit to achieve high temporal stability. The convenient adjustment of reference and object beams makes optical system compact and low-cost. The membrane fluctuations and qualitative phase are measured to demonstrate the capability and applicability of the system.

Citation Download Citation

Anuj Saxena, Azeem Ahmad, Vishesh Dubey, Sathi Das, Anowarul Habib, Balpreet Singh Ahluwalia, and Dalip Singh Mehta "Highly temporal phase stable common-path quantitative phase microscope", Proc. SPIE 12630, Advances in Microscopic Imaging IV, 126300P (9 August 2023); https://doi.org/10.1117/12.2671032

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