The development of an electrochemical immunoassay to detect digoxin in whole blood via hybridization of DNA

Ziyi Ning; Qiao Lin; Xueke Bai

doi:10.1117/12.2668994

24 March 2023 The development of an electrochemical immunoassay to detect digoxin in whole blood via hybridization of DNA

Ziyi Ning, Qiao Lin, Xueke Bai

Author Affiliations +

Proceedings Volume 12611, Second International Conference on Biological Engineering and Medical Science (ICBioMed 2022); 1261124 (2023) https://doi.org/10.1117/12.2668994
Event: International Conference on Biological Engineering and Medical Science (ICBioMed2022), 2022, Oxford, United Kingdom

Abstract

The creation of sensing technology that allows for the direct, quick and accurate detection of small molecules in whole blood makes a significant influence on global health since it allows people to process illness detection, surveillance, as well as at-home care. When a free DNA strand is bound to target molecules, it can be sterically prevented from hybridizing with its complementary surface-bound strand. CeSHHA is a competitive eSHHA (electrochemical steric hindrance hybridization assay) that allows for the small molecules with direct quantitative detection in complicated matrices like soil sample or whole blood. The significance of this low-cost test for applications such as point-of-care diagnosis and therapy monitoring is discussed in the report. Monitoring the concentration of digoxin in human blood to avoid the toxicity of drugs using CeSHHA is a good example to show merit of this assay during detecting small molecules.

Citation Download Citation

Ziyi Ning, Qiao Lin, and Xueke Bai "The development of an electrochemical immunoassay to detect digoxin in whole blood via hybridization of DNA", Proc. SPIE 12611, Second International Conference on Biological Engineering and Medical Science (ICBioMed 2022), 1261124 (24 March 2023); https://doi.org/10.1117/12.2668994

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available