Paper
20 September 2023 Hydrodynamics mediated interactions between live active rotors in dual optical tweezers
Author Affiliations +
Abstract
We report on the interactions between internally driven pairs of active rotors in a dual optical tweezer. The active rotor is Bacillus subtilis, a wild-type, gram- positive bacterium that uses flagellar rotation for motility. A pair of bacteria are held at different distances and their respective flagellar rotations are studied through the durations of their approach and retraction from each other. The aim of our work is to investigate the nature of the interactions between two active confined rotors trapped in their pristine form. We find, that the frequency of the rotating flagella decreases in both confined bacteria on approaching each other and increases when retracted from each other. In other words, the flagellar rotations of a bacterium slow down while in the presence of a nearby neighbor and speeds up as the neighbor retreats. Our results show a similar trend as when compared to free swimming bacteria wherein they avoid each other on approach through modulation of their rotating flagella. We investigate through this setup the hydrodynamics mediated coupling between two such active rotors.
© (2023) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Ashwini V. Bhat, Naveena C. S., and Sharath Ananthamurthy "Hydrodynamics mediated interactions between live active rotors in dual optical tweezers", Proc. SPIE 12606, Optical Manipulation and Structured Materials Conference, 126061F (20 September 2023); https://doi.org/10.1117/12.3008383
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KEYWORDS
Bacteria

Optical tweezers

Dichroic mirrors

Scattered light

Video microscopy

Objectives

Physics

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