Research on the key technology of nucleic acid detection device

Luyang Duanmu; Yuanhua Yu; Hongzhuang Guo

doi:10.1117/12.2604513

31 December 2021 Research on the key technology of nucleic acid detection device

Luyang Duanmu, Yuanhua Yu, Hongzhuang Guo

Proceedings Volume 12065, AOPC 2021: Optical Sensing and Imaging Technology; 1206547 (2021) https://doi.org/10.1117/12.2604513
Event: Applied Optics and Photonics China 2021, 2021, Beijing, China

Abstract

With the rapid development of nucleic acid molecule detection technology, nucleic acid detection devices have become a global research hotspot in recent years. This paper uses multidisciplinary fusion technology to propose a nucleic acid fluorescence detection design scheme. The device is mainly composed of a motion module and an optical system. Among them, the motion module uses an S-type variable acceleration control method to precisely control the movement of the motor. The stable and accurate operation lays the foundation for the collection of fluorescent signals; the optical system uses LED as the fluorescent excitation light source. The LED collimated uniform light path is designed. Use image stitching to stitch the collected images into a complete image for analysis. Finally, measure the operating error of the motor, the average error does not exceed 14um; simulate the fluorescence signal acquisition system and collect the image of the fluorescent film, the image fluorescence is uniform, which verifies the rationality of the fluorescence acquisition system; collects simulated samples and blank control images , Analyze the stitched images, and the results clearly distinguish the simulated samples and the control group.

Citation Download Citation

Luyang Duanmu, Yuanhua Yu, and Hongzhuang Guo "Research on the key technology of nucleic acid detection device", Proc. SPIE 12065, AOPC 2021: Optical Sensing and Imaging Technology, 1206547 (31 December 2021); https://doi.org/10.1117/12.2604513

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