Sequence-dependent dynamics of DNA

David P. Millar; Larry C. Sowers

doi:10.1117/12.17768

1 May 1990 Sequence-dependent dynamics of DNA

David P. Millar, Larry C. Sowers

Proceedings Volume 1204, Time-Resolved Laser Spectroscopy in Biochemistry II; (1990) https://doi.org/10.1117/12.17768
Event: OE/LASE '90, 1990, Los Angeles, CA, United States

Abstract

The sequence-dependent high-frequency motion of a fluorescent base in DNA has been studied by picosecond time-resolved fluorescence depolarization measurements on a series of defined-sequence synthetic oligonucleotides of the type d[GCCXCCG] d[CGG(AP)GGC], where X = T, A, G or C and AP is the fluorescent adenine analogue 2- aminopurine. The fluorescence anisotropy contained a rapidly decaying component that was due to restricted internal rotation of AP and a slowly decaying component due to overall tumbling. The tumbling correlation time varied with X in a manner that reflected the association state of the oligonucleotide strands. The internal motion dynamics of AP depended on the nature of the base-pairing interactions between AP and x.

Citation Download Citation

David P. Millar and Larry C. Sowers "Sequence-dependent dynamics of DNA", Proc. SPIE 1204, Time-Resolved Laser Spectroscopy in Biochemistry II, (1 May 1990); https://doi.org/10.1117/12.17768

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