FIBI: a direct-to-digital microscopy approach for slide-free histology

Farzad Fereidouni; Taryn Morningstar; Alexander Borowsky; Richard Levenson

doi:10.1117/12.2612748

4 April 2022 FIBI: a direct-to-digital microscopy approach for slide-free histology

Farzad Fereidouni, Taryn Morningstar, Alexander Borowsky, Richard Levenson

Proceedings Volume 12039, Medical Imaging 2022: Digital and Computational Pathology; 1203903 (2022) https://doi.org/10.1117/12.2612748
Event: SPIE Medical Imaging, 2022, San Diego, California, United States

Abstract

Anatomic pathology is still the gold standard for tissue-based disease diagnosis, is centered on interpretation of H&E-stained sections on glass slides, a process requires labor-intensive and time-consuming specimen processing. We propose a simple, clinically relevant solution, termed FIBI (fluorescence imitating brightfield imaging), for directly creating diagnostic-quality images from unsectioned, fresh or fixed tissue specimens. FIBI can generate full-color histology-grade images within minutes. We have demonstrated the validity of this method by collecting 50 tissue samples from various organs and pathologies and comparing the diagnosis obtained using FIBI images with those determined from adjacent, conventionally prepared H&E-stained slides.

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Citation Download Citation

Farzad Fereidouni, Taryn Morningstar, Alexander Borowsky, and Richard Levenson "FIBI: a direct-to-digital microscopy approach for slide-free histology", Proc. SPIE 12039, Medical Imaging 2022: Digital and Computational Pathology, 1203903 (4 April 2022); https://doi.org/10.1117/12.2612748

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KEYWORDS

Microscopy

Tissues

Pathology

Glasses

Gold

Luminescence

Natural surfaces

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