Visualizing the anti-cancer effects of chrysin nanoparticles by flow cytometry, microscopy and Fourier transform infrared spectroscopy

Siya Kamat; Madhuree Kumari; C. Jayabaskaran

doi:10.1117/12.2621529

3 March 2022 Visualizing the anti-cancer effects of chrysin nanoparticles by flow cytometry, microscopy and Fourier transform infrared spectroscopy

Siya Kamat, Madhuree Kumari, C. Jayabaskaran

Author Affiliations +

Proceedings Volume 11977, Colloidal Nanoparticles for Biomedical Applications XVII; 1197707 (2022) https://doi.org/10.1117/12.2621529
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Nano formulations of anti-cancer natural products have revolutionized anti-cancer therapy and its challenges such as short half-life, low therapeutic index, non-specificity, efficiency, and bioavailability. Chrysin (Chr), a dihydroxyflavone isolated from a marine fungus Chaetomium globosum, is a known anti-cancer natural product. However, its free use in clinical scenarios is disadvantaged due to poor water solubility, rapid metabolism, and non-specific toxicity. In this study, Chr-nanoparticles (NPs) were prepared by the emulsion-diffusion evaporation technique. The optimized formulation of Chr-NPs was characterized for zeta size, potential, polydispersity index, transmission electron microscopy (TEM), and Fourier Transform Infrared Spectroscopy (FTIR). Chr-NPs had an average zeta size of 217.93 ± 3.45 nm, and a zeta potential of -15.63 ± 3.9 mV. As seen under the TEM, Chr-NPs measured an average size of 98.55 ± 4.01 nm. The NPs demonstrated an initial burst followed by a sustained release behaviour. The anti-cancer effects of Chr-NPs were investigated in human cervical cancer cells (HeLa) and human embryonic kidney (HEK) cells. MTT and propidium iodide live/dead flow cytometry assays demonstrated an improved IC50 value over the free drug treatment. An interplay of apoptosis and autophagy cell death mechanisms in Chr-NP treated HeLa cells was observed by Acridine Orange and MDC staining, visualized by confocal microscopy. Chr-NPs induced structural changes in the HeLa cell surface proteins and lipids were studied by FTIR spectroscopy. Natural products are the largest source of drug discovery; this study can pave the way to improve their chemistry sustainably and explore the cell death mechanisms.

Conference Presentation

Citation Download Citation

Siya Kamat, Madhuree Kumari, and C. Jayabaskaran "Visualizing the anti-cancer effects of chrysin nanoparticles by flow cytometry, microscopy and Fourier transform infrared spectroscopy", Proc. SPIE 11977, Colloidal Nanoparticles for Biomedical Applications XVII, 1197707 (3 March 2022); https://doi.org/10.1117/12.2621529

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