Super-resolution imaging of the third dimension of the actin cytoskeleton using metal-induced energy transfer and micropatterning

Carolin Grandy; Fabian Port; Jonas Pfeil; Kay-Eberhard Gottschalk

doi:10.1117/12.2608066

2 March 2022 Super-resolution imaging of the third dimension of the actin cytoskeleton using metal-induced energy transfer and micropatterning

Carolin Grandy, Fabian Port, Jonas Pfeil, Kay-Eberhard Gottschalk

Author Affiliations +

Proceedings Volume 11967, Single Molecule Spectroscopy and Superresolution Imaging XV; 1196709 (2022) https://doi.org/10.1117/12.2608066
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Cells adapt their actin cytoskeletons architecture to structural cues of the environment in all three dimensions. Nevertheless, how manipulating cell shape influences the actin cytoskeletons z-dimension is unstudied, but crucial for an understanding of the mutual influence of cell shape, cell tension and actin architecture. To study the effect of shape on the z-dimension of the actin cytoskeleton we combine metal-induced energy transfer as a super-resolution technique with micropatterning. This allows us not only to precisely manipulate the shape of the cell but also to regulate forces by changing the shape while studying specific actin structures with super-resolution.

Conference Presentation

Citation Download Citation

Carolin Grandy, Fabian Port, Jonas Pfeil, and Kay-Eberhard Gottschalk "Super-resolution imaging of the third dimension of the actin cytoskeleton using metal-induced energy transfer and micropatterning", Proc. SPIE 11967, Single Molecule Spectroscopy and Superresolution Imaging XV, 1196709 (2 March 2022); https://doi.org/10.1117/12.2608066

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available